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The term "nucleotide length" as used herein means the total number of the nucleotides (monomers) in a given sequence. The oligomer comprises a contiguous nucleotide sequence of from about 10 to about 50, such as 10-20, 16-20, 10-30, 10-35, 10-40, or 10-45 nucleotides in length. The term "polynucleotide" as used herein refers to two or more nucleotides linked in sequence. It will be recognized that when referring to a sequence of nucleotides or monomers, what is referred to is the sequence of bases, such as A, T, G, C or U, and analogs thereof. Such methods are helpful to reduce unnecessary killing of animals during testing of the molecule's toxicity and/or enhance the possibilities that the molecules will be safe for in vivo administration. Any other methods disclosed in this application and/or known in the art can further be combined with the calcium oscillation assay and/or the sequence score method. In another aspect, the disclosure provides a sequence score method to measure or predict toxicity of a molecule. In other aspects, the disclosure provides a combined method of using a calcium oscillation assay and a sequence score method.

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So he and his friends are gonna pick me up, we’ll have dinner in a Rock’n’Fellers restaurant and then stay at the mall before heading to the cinema. In certain embodiments, the invention provides a method for both selecting a molecule and then utilizing the molecule. In other embodiments, the present invention is also directed to a method of selecting or identifying a molecule having tolerable in vivo neurotoxicity by performing in vivo tolerability studies. The in vivo tolerability study can also be used after selecting a small number of candidate molecules after performing the calcium oscillation assay and/or sequence score calculation. Small molecules can comprise any therapeutic molecules that is not a peptide, a polypeptide, a protein, and a polynucleotide. In other embodiments, the molecules selected according to the present methods are therapeutic molecules. Therefore, for therapeutics, an animal or a human, suspected of having a disease or disorder can be treated by administering molecules in accordance with this disclosure.

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