The term "nucleotide length" as used herein means the total number of the nucleotides (monomers) in a given sequence. The oligomer comprises a contiguous nucleotide sequence of from about 10 to about 50, such as 10-20, 16-20, 10-30, 10-35, 10-40, or 10-45 nucleotides in length. The term "polynucleotide" as used herein refers to two or more nucleotides linked in sequence. It will be recognized that when referring to a sequence of nucleotides or monomers, what is referred to is the sequence of bases, such as A, T, G, C or U, and analogs thereof. Such methods are helpful to reduce unnecessary killing of animals during testing of the molecule's toxicity and/or enhance the possibilities that the molecules will be safe for in vivo administration. Any other methods disclosed in this application and/or known in the art can further be combined with the calcium oscillation assay and/or the sequence score method. In another aspect, the disclosure provides a sequence score method to measure or predict toxicity of a molecule. In other aspects, the disclosure provides a combined method of using a calcium oscillation assay and a sequence score method.



The disclosure also provides an in vivo tolerability assay that can be used separately or combined with the calcium oscillation assay and/or the sequence score method. Calcium oscillations can also regulate interactions of neurons with associate glia, in addition to other associated neurons in the network, to release other neurotransmitters in addition to glutamate. Networks of cortical neurons have been shown to undergo spontaneous calcium oscillations resulting in the release of the neurotransmitter glutamate. Back then, in the 'Stone Age' of police work, we didn't have computers so there were few ways of tracking crimes across jurisdictional lines. If you and your partner are thinking about joining a swinging community in your area, there are a few things you should know. Woman of all ages love this position due to the extreme pleasure it gives them from cliteral stimulation from there partner as well as intercourse. For non-Survivor fans, I would love to hear from you.



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The present disclosure also provides methods for selecting a molecule having reduced toxic side effects. The present methods comprise identifying molecules that have lower or reduced toxicity. In one embodiment, the toxicity, e.g., in vivo acute neurotoxicity, of the molecule is tested by measuring intracellular free calcium oscillations (calcium oscillations) in vitro in neuronal cells which are in contact or have been in contact with the molecule. Formerly known as wife swapping, sex dating girls is a lifestyle that involves couples or singles who choose to allow their partners to have sex with other people while still being committed to each other. But, no matter how much conversation you've had, you really don't know who is on the other end of the conversation. Hot and horny mature babes who want to bang younger guys are everywhere and all around. Non-limiting examples of the therapeutic molecules include oligomers, one or more nucleotides, one or more nucleosides, one or more amino acids, polynucleotides, peptides, proteins, polypeptides, or small molecule compounds that are naturally occurring, modified, recombinantly produced, or chemically synthesized.