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In an embodiment, the oscillation frequency can be determined by a time interval from commencement of a first oscillation in intracellular calcium flux to a commencement of a second oscillation in intracellular calcium flux. Calcium oscillations are important for the proper functions of neuronal cells. For example, detection of intracellular calcium flux can be achieved by staining the cells with fluorescent dyes which bind to calcium ions (known as fluorescent calcium indicators) with a resultant, detectable change in fluorescence (e.g., Fluo-4 AM and Fura Red AM dyes available from Molecular Probes. In one embodiment, the calcium oscillations measured in the present methods are the cumulative increase in calcium oscillations within a culture of neuronal cells, whereby the time to reach the maximum fluorescence signal constitutes the magnitude of the calcium response. In some embodiments, the calcium oscillations measured in the present methods are AMPA-dependent calcium oscillations. In some embodiments, calcium oscillations are measured in the present method through the use of fluorescent probes which detect the fluctuations of intracellular calcium levels. In one aspect, the disclosure sets forth a calcium oscillation assay as one way of measuring or predicting toxicity of a molecule. In some embodiments, the calcium oscillation assay can include a positive control cell (i.e., a cell exposed to a molecule that is known to reduce calcium oscillations to an untolerable level) or a negative control cell (i.e., a cell exposed to a molecule that is known not to affect calcium oscillations in the cell).

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