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When designing zinc finger proteins to recognize the CAG region, a set of 1- and two-finger modules might be employed in a `combine and match` mixture. To repress each alleles of the Htt (non-allele-particular), ZFPs were designed to bind to the Htt promoter and exon 1 region, wherein the target site was not entirely inside the CAG repeat. Eevie-like most of the models I spoke to for this article-broadcasts herself via the site MyFreeCams, or MFC. The ahead primer introduces an AvrII site, the reverse primer introduces a HindIII site. To detect expression from the wt mouse Htt allele, forward primer CAGGTCCGGCAGAGGAACC (SEQ ID NO:193) and reverse primer TTCACACGGTCTTTCTTGGTGG (SEQ ID NO:194) have been utilized in the actual-time RT-PCR; to detect expression from the knockin Htt allele, ahead primer GCCCGGCTGTGGCTGA (SEQ ID NO:195) and reverse primer TTCACACGGTCTTTCTTGGTGG (SEQ ID NO:196) were used. No Htt repression was noticed in the normal fibroblast line (CAG18/18 (SEQ ID NOS 245 and 245)). In contrast, glorious allelic discrimination was noticed within the CAG 15/67 (SEQ ID NOS 239 and 242) and CAG15/70 (SEQ ID NOS 239 and 240) strains at both a high and low dose of transfected 30640 mRNA; similar results were obtain for the two HD fibroblast lines with intermediate CAG repeat length on the mutant allele (CAG 18/forty four (SEQ ID NOS 245 and 244) and CAG 18/45 (SEQ ID NOS 245 and 243)).about.wherein the expanded allele is repressed by .about.80% at both the high and low doses of 30640, yet the CAG18 (SEQ ID NO: 245) allele remained unaffected.

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