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CD62L and negative for expression of CD45RA and/or CD45RO are expanded. APCs provided in WO 03/057171 and US2003/0147869 are hereby incorporated by reference in their entirety. Suitable costimulatory ligands further include target antigens, which may be provided in soluble form or expressed on APCs or aAPCs, that bind engineered TCRs or CARs expressed on modified T cells. Finally, the aAPCs provide an efficient platform to expand genetically modified T cells and to maintain CD28 expression on CD8 T cells. In a particular embodiment K32 or U32 aAPCs are used to direct the display of one or more antibody-based stimulatory molecules on the AAPC cell surface. Populations of T cells can be expanded by aAPCs expressing a variety of costimulatory molecules including, but not limited to, CD137L (4-1BBL), CD134L (OX40L), and/or CD80 or CD86. In various embodiments, expanded T cell populations comprise one or more of the following phenotypic markers: CCR7, CD3, CD4, CD8, CD27, CD28, CD62L, CD95, CD122, CD127, and HLA-DR. T lymphocytes characterized by the expression of phenotypic markers of naive T cells including CD62L, CCR7, CD28, CD27, CD122, and CD127 are expanded. Illustrative examples of CD2 binding agents include, but are not limited to, CD2 ligands and anti-CD2 antibodies, e.g., the T11.3 antibody in combination with the T11.1 or T11.2 antibody (Meuer, S. C. et al.

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