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The portion of methylated DNA is then defined as: 1 − ((HpaII/EcoRI) / (MspI/EcoRI)). Susumu Ohno hypothesized that, through evolution of sex chromosomes, transcription from the X was upregulated to compensate for the degeneration of the Y (Ohno 1967 Adler et al. 2011), a approach that strongly influences evolution of the genome. Only 129 contigs partially aligned to the genome (considerably less than 25% of the contig size aligned 10.15 Mb) and 148 contigs did not align at all to the genome (3.58 Mb). The increased assembly duration was mainly owing to heterozygous loci currently being separated into personal alleles (haplotigs). We aligned all Ensembl predicted X chromosome coding locations outside of the pseudoautosomal location to the Y chromosome reference assembly to estimate divergence. He predicted that dosage compensation in XX females (i.e., the downregulation of X transcription in mammals) evolved to counter X upregulation. Combined with the observation that extremely sexual intercourse-biased genes are largely expressed in the gonad, differential distribution of sexual intercourse-biased genes reflects variances in evolutionary pressures joined to tissue-certain regulation of X chromosome transcription. For a lot easier interpretation of the outcomes, common names were utilized in its place of formal gene symbols for a subset of genes (Erk2, Mek1, Fthl17e, Fthl17f, and H2al1m) in the figures and Additional file 3: Table S2.