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Fragment coverage at EwS-specific DHSs correlated well with the genetic approaches (Supplementary Figs. Applying our LIQUORICE software package on a set of ARMS-distinct DHSs that we described applying publically obtainable data61 (Supplementary Data 8), we in truth observed a characteristic reduction in cfDNA fragment coverage that was certain to ARMS samples (Fig. 5f). This investigation confirms that the LIQUORICE examination of cfDNA fragmentation designs generalizes over and above EwS, and it indicates that our approach could possibly be broadly practical for detecting and quantifying tumor-derived DNA unbiased of any genetic aberrations. Mean DNA methylation degrees across EwS-precise DHSs ended up quantified and plotted utilizing the MIRA v1.8.073 with the adhering to options: location size was set to 2000 bp, selection of bins for every region was established to 21, minBaseCovPerBin was set to 100, and the centre of the 2000 bp areas was utilised for plotting. Plasma and mobile parts were being separated by centrifugation at 1600_g for 10 min with gentle break and acceleration set to one. Plasma was centrifuged a next time for 10 min at 16,000_g at room temperature to take away any remaining mobile debris and stored at _80 °C right until the time of cfDNA extraction
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