BongaCams is one of the top sex cam sites for kinky adults. In one embodiment, the behavioral performance is measured by injecting the molecule to a mammal, e.g., a brain of a mammal, e.g., intracerebroventricular (ICV) or intrathecal (IT) administration, and grading the mammal's behavioral performance on a scale of 0 to 4. In certain embodiments, the behavioral score is less than or equal to the total score of 3, the total score of 2, the total score of 1, or the total score of 0. In some embodiments, the behavioral score is determined as described in Example 5 below. In some embodiments, the calcium oscillations of a molecule having intolerable in vivo neurotoxicity are less than 70%, 60%, free private sexcam 50%, 40%, 30%, 20%, 10%, 5%, or 1% of the calcium oscillations in a vehicle control cell. For NOR, data are analyzed using either a paired t-test for within-group analyses or by an ANOVA followed by a Dunnett's post-hoc test for between group analyses. Join our Facebook group or email us us to make ЈЈЈs by sharing your story.



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In certain embodiments, the animals are observed at about 0.5 hour, about 1 hour, about 1.5 hours, about 2 hours, about 2.5 hours, about 3 hours, about 3.5 hours, about 4 hours, about 4.5 hours or about 5 hours following the injection of the molecule. In certain embodiments, the animals are observed at 0.5 hour, 1 hour, 1.5 hours, 2 hours, 2.5 hours, 3 hours, 3.5 hours, 4 hours, 4.5 hours or 5 hours following the injection of a molecule. In certain embodiments, the present disclosure incudes a method of identifying or determining a molecule having intolerable in vivo neurotoxicity comprising measuring calcium oscillations in vitro in neuronal cells after being in contact with the molecule in the neuronal cells, wherein the calcium oscillations of the molecule is equal to or less than 50% of the calcium oscillations in a vehicle control cell. The present methods can further comprise measuring a behavioral performance of an animal by a molecule. In some embodiments, the in vivo tolerability score is measured by administering the molecule to a mammal, e.g., to the brain of the mammal, e.g., via intracerebroventricular (ICV) administration or intrathecal (IT) administration.