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FIG. 15 shows activity (% NHEJ) of ZFNs including linkers as described herein and including modified junctions as between the linker and cleavage domain (in the left and/or right ZFNs of the pair as indicated) (SEQ ID NOs:280-303 and 51, respectively, in order of appearance). 1. A fusion protein comprising a DNA-binding domain having an N-terminus and a C-terminus, wherein the DNA-binding domain binds to a nucleotide target site; a truncated FokI cleavage domain in which the N-terminal residues QLVKS (residues 158 to 162 of SEQ ID NO:1) are deleted; and a linker between the C-terminus of the DNA-binding domain and the N-terminus of the cleavage domain, wherein the linker comprises a sequence selected from the group consisting of (N)GICPPPRPRTSPP (L8a, SEQ ID NO:2); (T)GTAPIEIPPEVYP (L8b, SEQ ID NO:3); (N)GSYAPMPPLALASP (L8c, SEQ ID NO:4); (P)GIYTAPTSRPTVPP (L8d, SEQ ID NO:5); (N)GSQTPKRFQPTHPSA (L8e, SEQ ID NO:6); TGLMPPSHPRQPIHINF (L8g, SEQ ID NO:234); TGTVHTSPICPQTYP (L8i, SEQ ID NO:161); TGSGTPTRPHPPLPP (L8j, SEQ ID NO:235); (H)LPKPANPFPLD (L7-1, SEQ ID NO:7); (H)RDGPRNLPPTSPP (L7-2, SEQ ID NO:8); (H)RLPDSPTALAPDTL (L7-6, SEQ ID NO:9); (D)PNSPISRARPLNPHP (L7-3, SEQ ID NO:10); (Y)GPRPTPRLRCPIDSLIFR (L7-5, SEQ ID NO:11); (H)CPASRPIHP (L6-2, SEQ ID NO:12); (G)LQSLIPQQLL (L6-6, SEQ ID NO:13); (G)LQPTVNHEYNN (L6-7, SEQ ID NO:14); and (P)ANIHSLSSPPPL (L6-1, SEQ ID NO:15) and further wherein the amino acid residue shown in round parentheses is optionally present.

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As we have discussed above, the Nguni people have populated the areas of South Africa earlier than has been suspected, and their culture, no matter how this has been spun by historians and detractors of African culture, customs, traditions and practices are the same and have been so as far as the literature on this subject can reach into antiquity. As noted with Queen Modjadji 'the rainmaker', such persons and practices are found all around the various clans that form the Nguni/Bakone peoples, their customs, traditions, culture and practices, which are one and the same, as both Hubs have been asserting. To put together a coherent historical matrix and mosaic of Africans in South Africa, we need to interconnect all the various historical timelines and oral and traditional history as has already been begun to be sculpted in the first Hub- "South African Culture, Customs, Practices Writ Large: Re-Morphed Cultural Renaissance Against Dysfunctional Existence".

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