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Adjacent to the 5' LTR are sequences necessary for reverse transcription of the genome (the tRNA primer binding site) and for efficient packaging of viral RNA into particles (the Psi site). The U5 region is the sequence between the primer binding site and the R region and contains the polyadenylation sequence. The LTR contains numerous regulatory signals including transcriptional control elements, polyadenylation signals and sequences needed for replication and integration of the viral genome. Where reference is made herein to elements such as cloning sites, promoters, regulatory elements, heterologous nucleic acids, etc., it is to be understood that the sequences of these elements are present in RNA form in the lentiviral particles of the invention and are present in DNA form in the DNA plasmids of the invention. Thus, as used herein, the terms "packaging sequence," "packaging signal," "psi" and the symbol ".PSI.," are used in reference to the non-coding sequence required for encapsidation of retroviral RNA strands during viral particle formation.

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