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1989) Molecular Cloning A Laboratory Manual (2nd ed.; Cold Spring Harbor Laboratory Press); Sambrook et al., ed. 1987) Gene Transfer Vectors For Mammalian Cells, (Cold Spring Harbor Laboratory); Wu et al., eds., Methods In Enzymology, Vols. 1992) Molecular Cloning: A Laboratory Manual, (Cold Springs Harbor Laboratory, NY); D. N. Glover ed., (1985) DNA Cloning, Volumes I and II; Gait, ed. The still cold of the quad reminded Aegle of home. In one embodiment, random therapeutic molecules comprising nucleotide sequences (e.g., oligomers) targeting certain regions of pre-mRNA or mRNA encoding MAPT, BASP1, or APP are prepared to test their toxicities. A further example of a target nucleic acid sequence of the oligomers is APP pre-mRNA or APP mRNA. In some embodiments, the present method is utilized to select any therapeutic molecules comprising a nucleotide sequence (e.g., oligomers) that hybridize to a region within a MAPT transcript, e.g., SEQ ID NO: 2 (SEQ ID NO: 2 can be mRNA if "t" is replaced with "u"), a BASP1 transcript or an APP transcript. APP cDNA which corresponds to APP mRNA is known as GenBank Accession No. Y00264.

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