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Short term recognition memory can be measured using the novel object recognition (NOR) task. In one embodiment, the method comprises a behavioral test score, which can be measured by administering the molecule to a mammal and grading the mammal's behavioral performance. The calcium oscillation assay and/or the sequence score method can be especially useful for screening numerous candidate molecules to determine their in vivo neurotoxicities. For example, long term toxicities can be determined by measuring the change in tubulin intensity in a cell by the molecule when the cell comes in contact with a molecule. Examples of assays measuring the change in tubulin intensity in a cell are provided below. In certain embodiments, the change in tubulin intensity is measured along with one or both of the change in calcium oscillation, sequence score, and/or in vivo tolerability assay. In some embodiments, the tubulin intensity in a cell that is not exposed to the tested molecule is referred to as the tubulin intensity in a control cell.

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