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The present disclosure also provides a method for selecting a molecule having tolerable in vivo acute neurotoxicity comprising measuring calcium oscillations in vitro in neuronal cells in vitro which are in contact with the molecule, wherein the molecule exhibits a calcium oscillation level comparable to a control. E5. A method of selecting or identifying a molecule having tolerable in vivo acute neurotoxicity comprising measuring calcium oscillations in vitro in neuronal cells which are in contact with the molecule, wherein the neuronal cells in contact with the molecule exhibit calcium oscillations at a level comparable to or higher than that of vehicle control cells. In other embodiments, the present disclosure provides a method of selecting a molecule having tolerable in vivo acute neurotoxicity comprising measuring in vivo tolerability. E27. The method of any one of embodiments 25 to 26, wherein the mRNA is expressed in a cell. Determination of the most effective therapeutic molecules usually involves synthesizing a large number of molecules designed to target a factor in a cell and testing that large number of molecules for activity and toxicity.

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