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Small molecule inhibition of spontaneous calcium oscillations mediated by either NMDA or AMPA will be assessed. Therapeutic protein inhibition of spontaneous calcium oscillations mediated by either NMDA or AMPA will be assessed. Addition of small molecule will inhibit AMPA receptor mediated oscillations. Data presented in FIG. 1 show that, addition of the AMPA receptor antagonist, 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX; 3 .mu.M), reduced calcium oscillations by 20% representing the total AMPA response in the assay (FIG. 1AMPA labeled bar shown). Tau antisense oligonucleotide impact on spontaneous calcium oscillations in primary neurons is shown in FIG. 4. The oligomers in neuronal cells showing AMPA-mediated oscillations that are equal to or greater than 75% of the calcium oscillations in the untreated control cells were selected for further analysis. The effect of therapeutic proteins, such as antibodies or antigen-binding fragments thereof, fusion proteins, cytokines, cell surface receptors, hormones or growth factors, on calcium oscillations will be measured using substantially the same method provided in Example 2. To measure primary cortical neuron spontaneous calcium oscillation, rat primary cortical neurons will be prepared.

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