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Antisense oligomer inhibition of spontaneous calcium oscillations mediated by either NMDA or AMPA was assessed in the presence or absence of 1 mM MgCl.sub.2 (representing 100% control in each case). FIG. 2: Graph showing inhibition of AMPA mediated calcium oscillations by antisense oligomers as an indication of neuronal network activity disruption. The ASO and other oligomerss that behaved similarly, were shown to negatively impact central nervous system (CNS) network activity in vivo and electrophysiologic spontaneous neuronal activity in vitro (data not shown). Calcium oscillations were reduced further, by about 80%, when N-methyl-D-aspartate (NMDA) receptor function was blocked by 1 mM MgCl.sub.2 (NMDA labeled bar shown). E81. In a method of testing or determining in vivo neurotoxicity of a molecule, the improvement comprising measuring calcium oscillations in vitro in neuronal cells which are in contact with the molecule. E45. The method of any one of embodiments 1 to 44, further comprising measuring tubulin intensity of the molecule in a culture of neuronal cells. E63. A method of testing or determining in vivo acute neurotoxicity of a molecule comprising the steps of 1) adding the molecule to a culture of neuronal cells and 2) measuring calcium oscillations in vitro in the neuronal cells.

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