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Therapeutic protein inhibition of spontaneous calcium oscillations mediated by either NMDA or AMPA will be assessed. Addition of therapeutic protein will inhibit AMPA receptor mediated oscillations. FLIPR signal was read for an additional 200 seconds (1 reading/second) after the addition of oligomer. Baseline signal (measurement of intracellular calcium) was read for 100 seconds (1 reading/second) before the addition of anti-sense oligomers. A second 5 minute post addition plate read (300 one second points) on the FLIPR was conducted to allow for additional data capture. Raw data from the 5 minute read was exported and, using Excel, spike amplitude and frequency was calculated. Data presented in FIG. 1 show that, addition of the AMPA receptor antagonist, 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX; 3 .mu.M), reduced calcium oscillations by 20% representing the total AMPA response in the assay (FIG. 1AMPA labeled bar shown). Addition of small molecule will inhibit AMPA receptor mediated oscillations. Small molecule inhibition of spontaneous calcium oscillations mediated by either NMDA or AMPA will be assessed.

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