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E23. The method of any one of embodiments 16 to 22, wherein the polynucleotide is an antisense oligonucleotide (i.e., oligomer) of about 10 to about 50 nucleotides in length. E24. The method of embodiment 23, wherein the antisense oligonucleotide modulates an expression of a target protein. E31. The method of any one of embodiments 23 to 30, wherein the antisense oligonucleotide is complementary to an mRNA or a pre-mRNA of the target gene. The term "complement" as used herein indicates a sequence that is complementary to a reference sequence. It will be recognized that when referring to a sequence of nucleotides or monomers, what is referred to is the sequence of bases, such as A, T, G, C or U, and analogs thereof. The term "design" or "oligomer design" or "ASO Sequence" as used herein refers to a pattern of nucleotides (e.g., DNA) and nucleotide analogs (e.g., LNA) in a given sequence. Exemplary polynucleotides can comprise a nucleotide sequence having two nucleotides, three nucleotides, four nucleotides, five nucleotides, six nucleotides, seven nucleotides, eight nucleotides, nine nucleotides, ten nucleotides, oligonucleotides, 50 nucleotides, 51 nucleotides, or more. The term "nucleic acids," "nucleotides," "nucleotide sequence," or "nucleic acid sequence" is intended to encompass plural nucleic acids (e.g., two or more, three or more, etc.).

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