E37. The method of any one of embodiments 34 to 36, wherein the tolerability category comprises at least one, at least two, at least three, at least four, or at least five tolerability categories. The present invention provides methods where the in vivo tolerability is graded into one of five tolerability categories. E66. The method of any one of Embodiments 63 to 65, wherein the calcium oscillations are AMPA receptor dependent. E59. The method of any one of embodiments 1 to 58, wherein when the molecule is administered to laboratory animals, more than 85% of the animals survive. E45. The method of any one of embodiments 1 to 44, further comprising measuring tubulin intensity of the molecule in a culture of neuronal cells. E35. The method of embodiment 33, wherein the molecule is administered to the brain of the mammal. E34. The method of embodiment 33, wherein the in vivo tolerability is measured by administering the molecule to a mammal and grading the mammal's tolerability in a tolerability category. 15. The method of claim 14, wherein the behavioral test is a short term memory test, a spatial learning and memory test, a gait analysis test, or any combination thereof.



E43. The method of embodiment 42, wherein the behavioral test score is measured by administering the molecule to a mammal and grading the mammal's` behavioral performance. E2. The method of embodiment 1, wherein the calcium oscillations of the molecule are compared to the calcium oscillations in neuronal cells that are not exposed to the molecule ("control cells"). It is understood that wherever aspects are described herein with the language "comprising," otherwise analogous aspects described in terms of "consisting of" and/or "consisting essentially of" are also provided. Thus, the term "and/or" as used in a phrase such as "A and/or B" herein is intended to include "A and B," "A or B," "A" (alone), and "B" (alone). The term "oligomer" in the context of the present invention, refers to a molecule formed by covalent linkage of two or more nucleotides (i.e., an oligonucleotide). It is to be noted that the term "a" or "an" entity refers to one or more of that entity; for example, "a molecule," is understood to represent one or more molecules. E57. The method of any one of embodiments 1 to 56, wherein when the molecule is administered to laboratory animals, more than 20% of the animals survive.



E9. The method of embodiment 8, wherein the protein comprises an antibody or antigen-binding fragment thereof, a fusion protein, a cytokine, a cell surface receptor, a hormone, a growth factor, or any combination thereof. Determination of the most effective therapeutic molecules usually involves synthesizing a large number of molecules designed to target a factor in a cell and testing that large number of molecules for activity and toxicity. Proteins that are therapeutic molecules include, but are not limited to, antibodies or antigen-binding fragments thereof, fusion proteins, cytokines, cell surface receptors, hormones, growth factors, or any combination thereof. 6. The method of claim 1, wherein the calcium oscillations are AMPA receptor-dependent calcium oscillations. E88. In the method of Embodiment 87, wherein the in vivo tolerability is measured by administering the molecule to a mammal and grading the mammal's tolerability in a tolerability category. E36. A method of testing or determining in vivo tolerability of a molecule comprising administering the molecule to a mammal and grading the mammal's tolerability in a tolerability category.



E51. The method of any one of embodiments 16 to 50, wherein the polynucleotide is an antisense oligonucleotide. E14. The method of one of embodiments 1 to 13, further comprising administering the molecule to a subject in need of treatment of a disease or condition. E46. The method of embodiment 45, wherein the tubulin intensity of the molecule is compared to the tubulin intensity in neuronal cells not exposed to the molecule. The above methods of the present invention can further comprise measuring tubulin intensity in a culture of neuronal cells, expression of a target protein, or behavioral performance of the molecule. 16. The method of claim 1, further comprising measuring tubulin intensity of the molecule in a culture of neuronal cells. A method of testing or determining in vivo acute neurotoxicity of a molecule comprising measuring calcium oscillations in vitro in neuronal cells which are in contact with the molecule. Provisional Application No. 62/279,610, filed Jan. 15, 2016, all of which are incorporated herein by reference in its entirety. The content of the electronically submitted sequence listing (Name: 3338.035PC03 SL.txt, Size: 339,610 bytes; and Date of Creation: Feb. 4, 2016) submitted in this application is incorporated herein by reference in its entirety.



The headings provided herein are not limitations of the various aspects of the disclosure, which can be had by reference to the specification as a whole. Amino acid sequences are written left to right in amino to carboxy orientation. Non-limiting examples of the therapeutic molecules include oligomers, one or more nucleotides, one or more nucleosides, one or more amino acids, polynucleotides, peptides, proteins, polypeptides, or small molecule compounds that are naturally occurring, modified, free cam websites recombinantly produced, or chemically synthesized. If so, there are many sources for satisfying erotic literature. Keep in mind that there is a hierarchy of value to these communication techniques. "I think part of the appeal among indie producers is that, due to the fierce competition and abundance of content on sites like ManyVids, there needs to be a way for their particular clip to stand out," she explained. They’re more commonly called "Free cam websites girls" or "web free cam websites models," and they’re part of a version of sex work that goes beyond looking for fleshy hashtags on Instagram. Looking to meet local swingers?