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Illustrative examples of retroviral-derived env genes which can be employed in the invention include, but are not limited to: MLV envelopes, 10A1 envelope, BAEV, FeLV-B, RD114, SSAV, Ebola, Sendai, FPV (Fowl plague virus), and influenza virus envelopes. As used herein, the term "packaging vector" refers to an expression vector or viral vector that lacks a packaging signal and comprises a polynucleotide encoding one, two, three, four or more viral structural and/or accessory genes. Viral particles are produced by transfecting a transfer vector into a packaging cell line that comprises viral structural and/or accessory genes, e.g., gag, pol, env, tat, rev, vif, vpr, vpu, vpx, or nef genes or other retroviral genes. For example, the infectious particles can be collected by cell lysis, or collection of the supernatant of the cell culture, as is known in the art. Methods of preparing viral stock solutions are known in the art and are illustrated by, e.g., Y. Soneoka et al.

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They know fine well who they’re talking to, and what their intentions are. Polynucleotides can be prepared, manipulated and/or expressed using any of a variety of well established techniques known and available in the art. Naturally i can imagine precisely a perfect heck which established over the responsibilities while using fagot Ernest Gordon levitt, it’s possible that not even others? Levitt, "Definition of an efficient synthetic poly(A) site", Genes & Development (1989); 3: 1019-1025. cited by applicant . Kay, M. A., "Adenoviral Vectors for Hepatic Gene Transfer in Animals." Chest (1997); 111: 138S-142S. cited by applicant . Naldini, L., "Lentiviruses as gene transfer agents for delivery to non-dividing cells", Curr Opin Biotechnol. Naldini, L. et al., "In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector", Science (1996); 272(5259): 263-267. cited by applicant . Maldarelli et al., "Identification of posttranscriptionally active inhibitory sequences in human immunodeficiency virus type 1 RNA: novel level of gene regulation", Journal of Virology (1991); 65(11): 5732-5743. cited by applicant .

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